poly(sss) heparin mimetics  (Mimetics)

Journal: Clinical Science (London, England : 1979)

Article Title: Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth

doi: 10.1042/CS20220245

Figure Lengend Snippet: The acute rise in the maternal plasma concentration of pro-inflammatory cytokines IL-6 and TNF-α confirms the induction of mIA following poly(I:C) treatment. Data are presented as mean + SEM; * P <0.05, ** P <0.01, *** P <0.001 vs vehicle control at same time point.

Article Snippet: Dams in the treatment group received a single intraperitoneal injection of the viral mimetic poly(I:C) (InvivoGen, San Diego, U.S.A., low molecular weight (LMW) form; catalogue number tlrl-picw, lot numbers PIW-39-01 and PIW-40-01) at a dose of 10 mg/kg body weight [ , ]; control dams were injected with an equivalent volume of vehicle (endotoxin-free 0.9% saline, InvivoGen).

Techniques: Concentration Assay

Journal: Clinical Science (London, England : 1979)

Article Title: Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth

doi: 10.1042/CS20220245

Figure Lengend Snippet: Both tissues exhibit up-regulated cytokine transcription following poly(I:C) treatment with effects that are tissue-, time- and cytokine-dependent. Data presented as mean + SEM. * P <0.05, ** P <0.01, *** P <0.001 vs vehicle control.

Article Snippet: Dams in the treatment group received a single intraperitoneal injection of the viral mimetic poly(I:C) (InvivoGen, San Diego, U.S.A., low molecular weight (LMW) form; catalogue number tlrl-picw, lot numbers PIW-39-01 and PIW-40-01) at a dose of 10 mg/kg body weight [ , ]; control dams were injected with an equivalent volume of vehicle (endotoxin-free 0.9% saline, InvivoGen).

Techniques:

Journal: Clinical Science (London, England : 1979)

Article Title: Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth

doi: 10.1042/CS20220245

Figure Lengend Snippet: Both tissues show stimulated Tlr3 transcription following poly(I:C) treatment, albeit with a different temporal pattern of response. Data presented as mean + SEM. * P <0.05, *** P <0.001 vs vehicle on same gestational day.

Article Snippet: Dams in the treatment group received a single intraperitoneal injection of the viral mimetic poly(I:C) (InvivoGen, San Diego, U.S.A., low molecular weight (LMW) form; catalogue number tlrl-picw, lot numbers PIW-39-01 and PIW-40-01) at a dose of 10 mg/kg body weight [ , ]; control dams were injected with an equivalent volume of vehicle (endotoxin-free 0.9% saline, InvivoGen).

Techniques:

Journal: Clinical Science (London, England : 1979)

Article Title: Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth

doi: 10.1042/CS20220245

Figure Lengend Snippet: At GD16 ( A–C ), poly (I:C) treatment caused a significant reduction in maternal plasma BCAA concentration, contrasting with the increase in placental tissue BCAA concentration whereas there was no change in yolk sac with a non-significant trend ( P =0.061) towards increased BCAA concentration in fetal brain. At GD21 ( D–F ), maternal plasma and fetal tissue BCAA concentrations were not significantly different between treatment groups. Data presented as mean + SEM. *** P <0.001 vs vehicle control.

Article Snippet: Dams in the treatment group received a single intraperitoneal injection of the viral mimetic poly(I:C) (InvivoGen, San Diego, U.S.A., low molecular weight (LMW) form; catalogue number tlrl-picw, lot numbers PIW-39-01 and PIW-40-01) at a dose of 10 mg/kg body weight [ , ]; control dams were injected with an equivalent volume of vehicle (endotoxin-free 0.9% saline, InvivoGen).

Techniques: Concentration Assay

Journal: Clinical Science (London, England : 1979)

Article Title: Maternal immune activation in rats induces dysfunction of placental leucine transport and alters fetal brain growth

doi: 10.1042/CS20220245

Figure Lengend Snippet: Maternofetal transport of 14 C-leucine demonstrated a biphasic response to poly(I:C)-induced mIA, with reduced transport at GD16 contrasting with the increased transport at GD21 compared with vehicle control. Data presented as mean + SEM. ** P <0.01, *** P <0.001 vs vehicle control.

Article Snippet: Dams in the treatment group received a single intraperitoneal injection of the viral mimetic poly(I:C) (InvivoGen, San Diego, U.S.A., low molecular weight (LMW) form; catalogue number tlrl-picw, lot numbers PIW-39-01 and PIW-40-01) at a dose of 10 mg/kg body weight [ , ]; control dams were injected with an equivalent volume of vehicle (endotoxin-free 0.9% saline, InvivoGen).

Techniques:

Journal: Molecular oral microbiology

Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells

doi: 10.1111/omi.12270

Figure Lengend Snippet: OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in type I interferon expression by RT-qPCR. Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test. All data analysis was performed using GraphPad Prism version 8.2.0 for Windows, GraphPad Software, La Jolla California USA, www.graphpad.com.

Article Snippet: OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours.

Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test, Software

Journal: Molecular oral microbiology

Article Title: Type I Interferon and Interferon-stimulated Gene Expression in Oral Epithelial Cells

doi: 10.1111/omi.12270

Figure Lengend Snippet: OKF6/Tert-1 cells were treated with 1 μg/ml poly(I:C) for up to six hours and measured for changes in interferon-stimulated gene (ISG) expression by RT-qPCR. Measured genes were divided into each ISG basal expression group, according to Table 2: Low (A), Medium (B), and High (C). Bar graphs and error bars represent the means and standard errors of measurement (SEM) of three independent experiments, each with three biological replicates per condition. *p < 0.05, **p < 0.005, and ***p < 0.0005 compared to 0h, using a two-tailed, unpaired Student’s t-test.

Article Snippet: OKF6/Tert-1 cells were treated with either 1 μg/ml double-stranded RNA mimetic poly(I:C) (Invivogen, tlrl-picw) in cell culture media for up to six hours.

Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test